self ligation (TaKaRa)
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TaKaRa
self ligation
Self Ligation, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 7354 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t4+ligation/T4+DNA+Ligase/bio_rxiv__64898__2026__05__09__724044-74-23-28
Average 97 stars, based on 7354 article reviews
Self Ligation, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 7354 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t4+ligation/T4+DNA+Ligase/bio_rxiv__64898__2026__05__09__724044-74-23-28
Average 97 stars, based on 7354 article reviews
self ligation - by Bioz Stars,
2026-09
97/100 stars
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Amplification:Article Title: Mouse medulloblastoma driven by CRISPR activation of cellular Myc Article Snippet: .. The U6 stuffer insert was amplified from Lenti-V6 using U6 Forward primer 5′-AATAATCAATGTCGA GAGGGCCTATTTCCCATGATTCCT-3′ and U6 Reverse primer 5′-ATGGGCCCTCGTCGACCAATTCCCACTCCTTTCAAGACC-3′. dCas9-VP160-T2A-Puro was released from pAC94-pmax-dCas9-VP160-T2A-Puro by EcoRI digestion and cloned into EcoRI digested pT2K-CaGGS-IRES-mCherry by Gibson Assembly . sgRNA-M5, -M7, and -M 9 were cloned individually into pT2K-U6-stuffer-CAGGS-IRES-CFP. pT2K-U6-stuffer-CAGGS-IRES-CFP was digested with BsmBI to remove the stuffer DNA and annealed guide RNAs were inserted using Article Title: Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytes Article Snippet: .. The amplified DNA fragments were subsequently cloned and inserted into the pcDNA3.1 vector by HindIII (NEB, R3104S) and XbaI (NEB, R0145S) dual digestion, followed by Article Title: Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytes. Article Snippet: .. The amplified DNA fragments were subsequently cloned and inserted into the pcDNA3.1 vector by HindIII (NEB, R3104S) and XbaI (NEB, R0145S) dual digestion, followed by Clone Assay:Article Title: Mouse medulloblastoma driven by CRISPR activation of cellular Myc Article Snippet: .. The U6 stuffer insert was amplified from Lenti-V6 using U6 Forward primer 5′-AATAATCAATGTCGA GAGGGCCTATTTCCCATGATTCCT-3′ and U6 Reverse primer 5′-ATGGGCCCTCGTCGACCAATTCCCACTCCTTTCAAGACC-3′. dCas9-VP160-T2A-Puro was released from pAC94-pmax-dCas9-VP160-T2A-Puro by EcoRI digestion and cloned into EcoRI digested pT2K-CaGGS-IRES-mCherry by Gibson Assembly . sgRNA-M5, -M7, and -M 9 were cloned individually into pT2K-U6-stuffer-CAGGS-IRES-CFP. pT2K-U6-stuffer-CAGGS-IRES-CFP was digested with BsmBI to remove the stuffer DNA and annealed guide RNAs were inserted using Article Title: Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytes Article Snippet: .. The amplified DNA fragments were subsequently cloned and inserted into the pcDNA3.1 vector by HindIII (NEB, R3104S) and XbaI (NEB, R0145S) dual digestion, followed by Article Title: Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytes. Article Snippet: .. The amplified DNA fragments were subsequently cloned and inserted into the pcDNA3.1 vector by HindIII (NEB, R3104S) and XbaI (NEB, R0145S) dual digestion, followed by Ligation:Article Title: Mouse medulloblastoma driven by CRISPR activation of cellular Myc Article Snippet: .. The U6 stuffer insert was amplified from Lenti-V6 using U6 Forward primer 5′-AATAATCAATGTCGA GAGGGCCTATTTCCCATGATTCCT-3′ and U6 Reverse primer 5′-ATGGGCCCTCGTCGACCAATTCCCACTCCTTTCAAGACC-3′. dCas9-VP160-T2A-Puro was released from pAC94-pmax-dCas9-VP160-T2A-Puro by EcoRI digestion and cloned into EcoRI digested pT2K-CaGGS-IRES-mCherry by Gibson Assembly . sgRNA-M5, -M7, and -M 9 were cloned individually into pT2K-U6-stuffer-CAGGS-IRES-CFP. pT2K-U6-stuffer-CAGGS-IRES-CFP was digested with BsmBI to remove the stuffer DNA and annealed guide RNAs were inserted using Article Title: Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytes Article Snippet: .. The amplified DNA fragments were subsequently cloned and inserted into the pcDNA3.1 vector by HindIII (NEB, R3104S) and XbaI (NEB, R0145S) dual digestion, followed by Article Title: Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytes. Article Snippet: .. The amplified DNA fragments were subsequently cloned and inserted into the pcDNA3.1 vector by HindIII (NEB, R3104S) and XbaI (NEB, R0145S) dual digestion, followed by Article Title: Bioinformatics analysis and transcriptional regulation of TORC1 gene through transcription factors NRF1 and Smad3 in bovine preadipocytes. Article Snippet: PCR amplicons were cloned into t vector pMD 19 (simple) (Takara, Japan) and digested with Sac I and Hind III restriction enzymes (Takara, Japan). .. The DNA was extracted from the gel through gel extraction kit (Omega, Inc, USA) and ligated through Article Title: Function and Transcriptional Regulation of Bovine TORC2 Gene in Adipocytes: Roles of C/EBPγ , XBP1 , INSM1 and ZNF263 Article Snippet: PCR amplicons were cloned into t vector pMD 19 (simple) (Takara, Japan) and digested with Sac I and Hind III restriction enzymes (Takara, Japan). .. The DNA was extracted from the gel with a gel extraction kit ((Omega Bio-tek, Norcross, GA, USA) and ligated through Article Title: Genetic variants in the TORC2 gene promoter and their association with body measurement and carcass quality traits in Qinchuan cattle Article Snippet: The PCR amplicons were cloned into pMD-19 T-Vector (Takara, Japan) and digested with Sac I and Hind III restriction enzymes (Takara, Japan). .. The haplotype DNA was then extracted from the gel through the E.Z.N.A gel extraction kit (Omega, Biotek, Inc, USA) and ligated through Plasmid Preparation:Article Title: Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytes Article Snippet: .. The amplified DNA fragments were subsequently cloned and inserted into the pcDNA3.1 vector by HindIII (NEB, R3104S) and XbaI (NEB, R0145S) dual digestion, followed by Article Title: Ring finger protein 213 regulates B-cell receptor signaling, metabolism, and development in B lymphocytes. Article Snippet: .. The amplified DNA fragments were subsequently cloned and inserted into the pcDNA3.1 vector by HindIII (NEB, R3104S) and XbaI (NEB, R0145S) dual digestion, followed by Gel Extraction:Article Title: Bioinformatics analysis and transcriptional regulation of TORC1 gene through transcription factors NRF1 and Smad3 in bovine preadipocytes. Article Snippet: PCR amplicons were cloned into t vector pMD 19 (simple) (Takara, Japan) and digested with Sac I and Hind III restriction enzymes (Takara, Japan). .. The DNA was extracted from the gel through gel extraction kit (Omega, Inc, USA) and ligated through Article Title: Function and Transcriptional Regulation of Bovine TORC2 Gene in Adipocytes: Roles of C/EBPγ , XBP1 , INSM1 and ZNF263 Article Snippet: PCR amplicons were cloned into t vector pMD 19 (simple) (Takara, Japan) and digested with Sac I and Hind III restriction enzymes (Takara, Japan). .. The DNA was extracted from the gel with a gel extraction kit ((Omega Bio-tek, Norcross, GA, USA) and ligated through Article Title: Genetic variants in the TORC2 gene promoter and their association with body measurement and carcass quality traits in Qinchuan cattle Article Snippet: The PCR amplicons were cloned into pMD-19 T-Vector (Takara, Japan) and digested with Sac I and Hind III restriction enzymes (Takara, Japan). .. The haplotype DNA was then extracted from the gel through the E.Z.N.A gel extraction kit (Omega, Biotek, Inc, USA) and ligated through Luciferase:Article Title: Bioinformatics analysis and transcriptional regulation of TORC1 gene through transcription factors NRF1 and Smad3 in bovine preadipocytes. Article Snippet: PCR amplicons were cloned into t vector pMD 19 (simple) (Takara, Japan) and digested with Sac I and Hind III restriction enzymes (Takara, Japan). .. The DNA was extracted from the gel through gel extraction kit (Omega, Inc, USA) and ligated through Article Title: Function and Transcriptional Regulation of Bovine TORC2 Gene in Adipocytes: Roles of C/EBPγ , XBP1 , INSM1 and ZNF263 Article Snippet: PCR amplicons were cloned into t vector pMD 19 (simple) (Takara, Japan) and digested with Sac I and Hind III restriction enzymes (Takara, Japan). .. The DNA was extracted from the gel with a gel extraction kit ((Omega Bio-tek, Norcross, GA, USA) and ligated through Article Title: Genetic variants in the TORC2 gene promoter and their association with body measurement and carcass quality traits in Qinchuan cattle Article Snippet: The PCR amplicons were cloned into pMD-19 T-Vector (Takara, Japan) and digested with Sac I and Hind III restriction enzymes (Takara, Japan). .. The haplotype DNA was then extracted from the gel through the E.Z.N.A gel extraction kit (Omega, Biotek, Inc, USA) and ligated through |